FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5239822
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG1048882.001747387602098TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA122000.043.5853961
CGTCCCG117800.043.06917635
ATCGGAA124050.043.0575452
TCGGAAG124050.042.9487233
ACACGTC122650.042.81525813
GTCCCGA117950.042.785536
CGTCTGA122950.042.78398516
CCCGATC116600.042.74058538
CGGAAGA125000.042.6603434
ACCCGTC120750.042.5945832
TCCCGAT118400.042.5658837
CCGTCCC119300.042.5465134
CACCCGT120900.042.46729731
CCGATCT117200.042.46418439
CCCGTCC120900.042.4486933
TCACCCG121250.042.3818230
CACGTCT123950.042.3662114
ACGTCTG124750.042.1486315
TCTCGTA120050.042.0378743
GCACACG125550.041.9875811