FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727707

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727707
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3614411
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG42630.11794452816793663TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA7550.036.091491
CTCGTAT7050.035.42448844
TCTCGTA7200.034.37398543
ACACGTC8300.032.7997813
ATCGGAA8400.032.409312
CGTCTGA8350.032.33392716
TCGGAAG8500.032.0280233
TCGTATG7900.031.3281945
ATCTCGT8400.029.99911542
CACGTCT9000.029.998714
ACGTCTG9100.029.91628315
CGGAAGA9600.028.5960714
AATCTCG8150.028.15828941
GCACACG10500.025.7131711
CACACGT10750.025.11518912
AGAGCAC14150.020.355018
GAGCACA15000.018.9015339
AGCACAC15500.018.4369810
CCAGTCA15050.018.08916926
GAAGAGC16000.017.7201886
Produced by FastQC (version 0.11.5)