FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727708

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727708
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4561586
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG54290.11901562307495683TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA8500.033.9167141
CTCGTAT7900.033.8910844
TCTCGTA8050.032.98007243
TCGTATG8500.032.02815645
TCGGAAG9250.031.1339153
CGTCTGA9400.030.63709416
ATCGGAA9600.030.4675542
ACACGTC9950.029.62195413
ATCTCGT9000.029.49884242
ACGTCTG10000.029.24885415
CGGAAGA10450.027.7758584
CACGTCT11100.026.35031714
AATCTCG10650.024.2948241
GCACACG12350.023.50110211
CACACGT13900.021.36606812
AGTCAAC14050.018.7359234
CCAGTCA17500.016.58542826
AGAGCAC18150.016.364078
CAGTCAC17600.016.10767627
GTCAACA17050.015.83515735
Produced by FastQC (version 0.11.5)