FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727709

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727709
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45337617
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG2441250.5384601488869607TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT313900.038.77415544
TCTCGTA301000.038.67974543
TCGTATG321900.037.97826445
ATCTCGT311250.037.45651242
CGTCTGA343850.036.98256716
ACACGTC346400.036.69083813
ACGTCTG358700.035.4828615
CACGTCT363950.034.93393314
GATCGGA375500.034.137971
AGTCAAC364200.033.83847834
TCGGAAG384450.033.5031363
ATCGGAA389300.033.1724322
CACACGT384400.033.16908612
CGGAAGA396050.032.628424
GCACACG391250.032.62811
AATCTCG361000.032.37581641
GTCAACA386850.031.95205135
TCTGAAC404550.031.51997818
CCAGTCA404550.031.02721626
CAGTCAC404500.031.01992827
Produced by FastQC (version 0.11.5)