FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727712

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727712
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6540367
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC345620.5284412938906945TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT42000.040.17680442
TCTCGTA42000.040.06966441
CGTATGC41800.039.9384344
ATCTCGT43000.039.03316540
TCGTATG43250.038.91158343
CGTCTGA46250.037.9442816
ACACGTC47100.037.54611613
ACGTCTG48350.036.5754315
CACGTCT49200.035.8520814
GTATGCC46950.035.79714645
AATCTCG48700.034.41840739
GCACACG51700.034.1619411
TCGGAAG52200.034.136433
CACACGT51750.034.12893312
CGGAAGA52500.033.9429244
ATCGGAA52550.033.8662572
GATCGGA52600.033.825681
AGTCACC52250.033.07132728
CAGTCAC55100.031.60695527
GTCACCT54750.031.56025529
Produced by FastQC (version 0.11.5)