FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727713

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727713
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9178976
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC170050.18526031661919587TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA34700.024.76994741
CTCGTAT34950.024.7205842
CGTATGC35300.024.34680744
ATCTCGT36600.023.60702940
TCGTATG36900.023.47441743
GTATGCC40550.021.24999845
CGTCTGA47600.019.6620116
GATCGGA49700.018.7086661
ACACGTC49850.018.68429413
ACGTCTG51000.018.61588915
CACGTCT52450.018.01545714
GATCTCG48550.017.98182139
GTCACAC48800.017.98093829
ATCGGAA52300.017.907742
TCGGAAG52050.017.9009043
CGGAAGA53800.017.5277864
GCACACG55850.016.71731211
CACACGT56600.016.5752912
CCAGTCA58750.015.47180726
CAGTCAC60000.015.14947727
Produced by FastQC (version 0.11.5)