FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727715

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727715
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10207045
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG464040.4546271717230599TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG58450.039.10471332
CTCGTAT58500.038.7674444
CGTCCGC59200.038.61081333
TCTCGTA55700.038.49463343
TCCGCAC59050.038.41275435
GTCCGCA59400.038.40562434
TCGTATG59600.038.27843545
AGTCACG61600.037.25970528
ACACGTC63800.037.09187713
GTCACGT61650.037.04698629
CCGCACA61450.036.98573736
CGTCTGA64100.036.9182816
ATCTCGT58150.036.9114542
CGCACAT58200.036.76935237
CACGTCC64150.035.77895731
TCACGTC64350.035.66775530
CATCTCG60100.035.5322241
TCGGAAG68850.034.997013
ACGTCTG67800.034.9367415
GATCGGA69550.034.7009621
Produced by FastQC (version 0.11.5)