FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3058948
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC530651.7347467168451376TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTCG74200.035.78214339
GTATGCC75200.035.60260845
TCTCGTA74900.035.59792741
CTCGTAT74900.035.56672342
CGTATGC75800.035.29111544
ATCTCGT75700.035.2514540
TCGTATG76400.035.07399743
ACCAATC77050.034.6051736
GACCAAT78750.034.17187535
ACTGACC80100.034.0420532
CCAATCT78400.033.9518937
GATCGGA86500.033.52581
CACTGAC81750.033.41000431
CTGACCA82400.032.9837133
GTCACTG82800.032.96077329
CAATCTC80650.032.92045638
TGACCAA82700.032.67362634
ATCGGAA89750.032.3368572
TCGGAAG89650.032.2341733
CGTCTGA87950.032.179816
Produced by FastQC (version 0.11.5)