FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727720

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727720
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10365564
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGATG21500.019.46352232
CGATGTA21700.019.18119634
CCGATGT22050.018.87673433
TATCTCG22300.018.46466339
CACCGAT23750.017.71442431
CTCGTAT26600.017.1709742
TCTCGTA27250.016.76195341
CGTATGC27800.016.75319544
GTCACCG26300.016.42530329
ATCTCGT29250.015.92353840
TCGTATG29600.015.88659643
GTATGCC31600.014.95195645
TCACCGA28850.014.81716830
CGTCTGA40650.013.89158316
ACACGTC41900.013.47715613
GATCGGA40400.013.2080521
TCGGAAG42850.013.0778983
ATCGGAA42550.013.0168922
ACGTCTG44200.012.928555515
CGGAAGA44250.012.4606934
Produced by FastQC (version 0.11.5)