FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9028953
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC145090.16069415800480963TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT29000.024.51748842
CGTATGC29350.024.30070344
TCACGAT28750.024.26165235
TCTCGTA29650.024.13244641
TCGTATG31000.023.15294643
ATCTCGT31700.022.50084940
CATCACG32000.021.9368733
ACGATCT32550.021.3603737
GTATGCC34300.020.9900745
CACGATC34300.020.13913336
GATCGGA42650.018.4220811
ATCACGA38250.018.41123834
CGTCTGA43750.018.35805116
ATCGGAA45050.017.6405542
ACACGTC44700.017.5149213
TCGGAAG45400.017.4981583
ACGTCTG46000.017.41119415
CGGAAGA48350.016.3839914
CACGTCT48000.016.3576414
GATCTCG44500.016.2309539
Produced by FastQC (version 0.11.5)