FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727723

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727723
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7771126
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATG254110.3269925104804632TruSeq Adapter, Index 27 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA30650.040.22706643
CTCGTAT30600.040.14574444
TATCTCG31900.038.72055441
TCGTATG32800.037.9332145
CGTCTGA35050.037.808316
ATCTCGT32750.037.71632442
ACACGTC35600.037.47698213
GATCGGA35800.037.1821141
ATCGGAA36800.036.3160442
ACGTCTG36850.035.96149415
CACGTCT37150.035.79221314
TCGGAAG38000.035.3468483
CGGAAGA38400.034.5722924
GCACACG38900.034.297711
CACACGT39900.033.60727312
AGAGCAC48550.027.668948
CCAGTCA49300.026.74406226
CAGTCAC49350.026.71679527
GAGCACA50250.026.643329
GAAGAGC52100.025.999576
Produced by FastQC (version 0.11.5)