FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14942306
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGTAGCCATCTCGTATG234560.15697710915570864TruSeq Adapter, Index 15 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA32250.034.94956243
CTCGTAT33800.033.87933344
TCGTATG34650.033.3079545
ATCTCGT35200.031.82881242
CATCTCG36400.030.7795141
CGTCTGA43200.027.1323716
GATCGGA44000.026.8633691
ACACGTC44700.026.12122713
ACGTCTG46150.025.34926615
ATCGGAA47700.024.9694962
TCGGAAG47700.024.7775383
CACGTCT48150.024.34306114
CGGAAGA49850.023.7088994
GCACACG50850.023.18323511
CACACGT54200.021.66730912
GTAGCCA54800.021.55323636
TGTAGCC61400.019.20069935
AGTCACC64100.018.63675328
GTCACCT64250.018.3840129
CTGTAGC64300.018.36971734
Produced by FastQC (version 0.11.5)