FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727734

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727734
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12609858
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATG2454791.9467229527882073TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCG8450.042.8593866
TCGTATG307100.040.73164745
CGTACGT314550.040.2450334
CCGTACG314850.040.2068433
ACCGTAC316600.039.96927632
GTACGTA315000.039.92627335
CTCGTAT312900.039.76093344
CGTAATC297100.039.7494938
TCTCGTA299200.039.74699843
ACGGCGA9000.039.7403227
GTCACCG317800.039.72335429
CACCGTA320000.039.534831
TACGTAA315550.039.5001236
CGTCTGA325300.039.4994716
ACGTAAT301150.039.43161837
ATCTCGT302550.039.30689642
ACGTCTG331050.038.8405815
AATCTCG306600.038.54690641
ACACGTC333750.038.54658513
CACGTCT334350.038.4437714
Produced by FastQC (version 0.11.5)