FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727739

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727739
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9685130
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATG761060.7858025653759939TruSeq Adapter, Index 21 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAT102700.037.96722437
TTCGGAA104200.037.48525636
CGTTTCG106050.037.44467533
GTTTCGG105650.037.33090234
TTTCGGA106300.036.93521535
CGGAATC105650.036.54523538
TCGTATG112300.035.88123745
GTCACGT113400.035.63348829
CTCGTAT112600.035.546644
AGTCACG119100.035.51494228
AATCTCG110400.035.50312441
TCTCGTA112700.035.47550243
ACGTTTC113100.035.34875532
CACGTTT114950.034.9755831
ATCTCGT115000.034.8449742
TCACGTT116200.034.59987330
GATCGGA130200.034.0319181
ATCGGAA134100.033.4619182
CGTCTGA133100.033.38331216
TCGGAAG134700.033.3669743
Produced by FastQC (version 0.11.5)