FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727740

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727740
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7468594
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATG1078571.4441406240585577TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT140150.038.769134
CGTAATC137700.038.57916638
CCGTACG141250.038.4034733
GTACGTA141550.038.3082335
ACGTAAT142300.037.9800937
TACGTAA142850.037.89686236
ACCGTAC144150.037.8939232
CACCGTA146900.037.27643231
GTCACCG147450.037.15488429
AATCTCG144850.036.95469741
TCGTATG148150.036.9484645
CTCGTAT148150.036.6910344
TCTCGTA148250.036.60581643
ATCTCGT151350.035.85748742
TCACCGT154050.035.63559730
GTAATCT151050.035.21441339
GATCGGA166350.035.0181471
ATCGGAA169800.034.3994752
TCGGAAG169800.034.3566063
CGGAAGA169850.034.2272344
Produced by FastQC (version 0.11.5)