FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727742

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727742
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18842799
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATG792330.42049485323279207TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG104450.034.72638741
GTCACGA107750.034.5155529
TCGTATG106750.034.18524645
CTCGTAT106300.034.1826244
TCTCGTA107550.033.8280743
ACGAGTG110150.033.70106532
AGTCACG111200.033.66725528
CACGAGT112150.033.04023731
GATCGGA121150.032.9316181
ATCTCGT110750.032.91246842
TCGGAAG124850.031.9432853
ATCGGAA125600.031.908262
ACACGTC123650.031.51276213
CGTCTGA122850.031.49821716
CGGAAGA127150.031.2946664
ACGTCTG128450.030.2125715
CACGTCT130300.029.83541114
GGATATC124450.029.27224738
GCACACG136350.028.62700311
CACACGT137300.028.39615612
Produced by FastQC (version 0.11.5)