FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727744

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727744
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19329755
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATG1523320.7880699988178845TruSeq Adapter, Index 27 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG187950.039.5180541
CTCGTAT190550.039.20015344
TCTCGTA192150.038.85122743
GATCGGA204400.038.6315081
TCGTATG195150.038.5293845
ATCTCGT196350.038.05525242
ATCGGAA209450.037.8398252
TCGGAAG209500.037.748723
CGTCTGA207950.037.59546316
ACACGTC209100.037.4962913
CGGAAGA213100.037.0687684
ACGTCTG214050.036.5976415
CACGTCT215850.036.42794414
GCACACG221750.035.3267411
CACACGT224950.034.784212
AGAGCAC243950.032.545378
CAGTCAC237550.032.5339727
CCAGTCA238850.032.46993326
GTCACAT236650.032.37256229
TCTGAAC243950.032.08442318
Produced by FastQC (version 0.11.5)