FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727747

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727747
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16953331
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG215760.12726702498759682TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGC32900.032.45305333
ACGTCCG33100.032.39316632
TCCGCAC33350.031.67744435
GTCCGCA33950.031.58204834
GATCGGA37000.030.7950441
CCGCACA35550.029.7184136
CGCACAT35500.029.56990637
CTCGTAT36300.028.75105544
AGTCACG37850.028.52628
TCTCGTA36850.028.1388243
GTCACGT38250.028.1096629
ATCGGAA41600.027.9847582
TCGGAAG41800.027.6237953
ACACGTC41600.027.2497813
CGTCTGA41950.027.07620416
TCCGATC11550.027.0677558
TCGTATG38800.026.83914445
CACGTCC40350.026.79660631
ATCTCGT40050.026.17148642
TCACGTC41500.025.9622730
Produced by FastQC (version 0.11.5)