FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727748

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727748
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24775144
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA12900.012.8072021
CTCGTAT13500.012.16362544
TCTCGTA13700.010.67251343
ATCTCGT15800.09.681025542
TCGTATG16800.09.51873145
ACCCGTG16450.09.43639832
CACCCGT16650.09.188560531
ATCGGAA18500.09.1299542
ACACGTC19650.08.470839513
TCGGAAG19800.08.302483
CCCGTGA19450.08.2121133
TCACCCG19900.07.914079730
CGTCTGA20650.07.73384716
CGTGAGA20950.07.624253335
CGTATGC8401.4551915E-117.239598345
CCGTGAG22500.06.79908234
ACGTCTG26200.06.610688715
CGGAAGA26600.06.5113984
CACGTCT28100.06.323798714
AATCTCG30150.06.117810741
Produced by FastQC (version 0.11.5)