FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14748153
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCATTCCATCTCGTATGC1506211.0212872079642787TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCATTCCATCTCGTATG921130.6245731245126085TruSeq Adapter, Index 1 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC178300.043.01370245
GATCGGA117200.040.455131
TCGTATG281850.027.07475744
CTCGTAT279650.027.00627943
ATCGGAA290800.026.7953741
ACACGTC288450.026.75734912
CGTCTGA288850.026.6820815
TCTCGTA259600.026.52692242
TCGGAAG293500.026.5093572
ACGTCTG291400.026.45630614
CGGAAGA295150.026.3230323
ATCTCGT261900.026.24242441
CACGTCT294350.026.20644613
ACTCGTA14350.026.18139542
GCACACG297250.025.95006810
CATCTCG266200.025.8677440
CACACGT298550.025.83707211
AGAGCAC313650.024.7942737
GTCACAT312400.024.59254828
CCAGTCA315650.024.54602225
Produced by FastQC (version 0.11.5)