FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727751

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727751
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16210680
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATG783090.48307042024147046TruSeq Adapter, Index 27 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG101300.039.13289645
CTCGTAT101550.038.9479444
TCTCGTA96750.038.64823543
TATCTCG97250.038.29443741
ATCTCGT99900.037.40707442
CGTCTGA112050.035.93528416
ACACGTC115100.034.94396213
ACGTCTG116400.034.59234215
CACGTCT121200.033.1481114
CACACGT129550.030.99422312
GCACACG129750.030.94606411
CCAGTCA135800.029.57870526
GTCACAT136500.029.27864829
TCTGAAC138400.029.14546618
CAGTCAC137750.029.09464327
GTCTGAA148350.027.59711817
CTCCAGT148600.026.97845324
CGGAAGA153550.026.7096164
CTGAACT151650.026.61346819
TCCAGTC152000.026.45543925
Produced by FastQC (version 0.11.5)