FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727753

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727753
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8265224
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC12100.031.60785545
GATCGGA10700.022.6302971
CTCGTAT17050.022.16748443
TCGTATG18050.021.18864444
TCTCGTA15850.021.00699442
ATCTCGT16400.020.5768541
ACACGTC19900.019.44353912
CGTCTGA21350.018.54493315
ATCGGAA21050.018.276251
CATCTCG19300.017.95038840
ACGTCTG22150.017.87513714
TCGGAAG22600.017.4273722
CACGTCT22600.017.32013513
CGGAAGA24450.016.0166873
GCACACG24600.015.72871610
CACACGT25750.015.20099411
CAAGGAC33650.012.16707832
CAGTCAC36500.011.464128526
CTAACCG1450.006840558710.8599434
AGAGCAC36900.010.851537
Produced by FastQC (version 0.11.5)