FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10675600
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATG660740.6189253999775188TruSeq Adapter, Index 21 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG91500.035.45476545
CGTTTCG92350.035.42773433
TCGGAAT92000.035.31892437
CTCGTAT92850.035.20612344
TTCGGAA93000.035.01173836
GTTTCGG93200.034.93562334
TCTCGTA93650.034.8333143
TTTCGGA94500.034.5742535
ATCTCGT95950.033.9520742
AATCTCG97100.033.2950941
CGGAATC96950.033.11454438
ACGTTTC100150.032.82578732
GTCACGT101150.032.71942529
AGTCACG104850.032.29498728
TCACGTT103700.031.97754330
CACGTTT103700.031.94073731
CGTCTGA120650.028.88354316
GAATCTC112550.028.82454340
GATCGGA121400.028.7683071
ACGTCTG123150.028.29719515
Produced by FastQC (version 0.11.5)