FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727757

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727757
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25765408
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG13900.06.967292345
CTCGTAT12000.06.74836644
ACCGAAC9707.2759576E-116.493789732
CCGAACT10905.456968E-126.398129533
TCTCGTA10852.910383E-116.219692743
CGAACTT12302.1827873E-115.852888634
GATCGGA13252.3646862E-115.63723951
ATCTCGT13951.200533E-105.32115842
GTCACCG15905.456968E-125.23554729
Produced by FastQC (version 0.11.5)