FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727758

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727758
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16836541
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC786970.4674178621368843TruSeq Adapter, Index 10 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT100700.037.66774742
TCTCGTA101500.037.3708641
TATCTCG101350.037.11539539
CGTATGC103500.036.92998544
ATCTCGT104400.036.31123440
TCGTATG105700.036.22519343
GTATGCC112350.034.06109645
CGTCTGA118650.033.99755516
ACACGTC122100.032.98107513
CACTAGC117150.032.95158431
ACGTCTG123800.032.5282815
CACGTCT127450.031.75548414
ACTAGCT122400.031.72211632
GCTTATC119850.031.64694436
GTCACTA124400.031.03377729
GATCGGA133100.030.9545211
TCGGAAG134100.030.81973
GCACACG132250.030.46719411
ATCGGAA136550.030.3526272
CGGAAGA136200.030.3445074
Produced by FastQC (version 0.11.5)