FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727763

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727763
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6243200
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC83270.1333771143003588TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCAAT19000.022.73868833
ACGCCAA19000.022.62007532
TATCTCG19650.021.98246839
TCTCGTA20400.021.39485441
CTCGTAT20950.021.04778142
TCGTATG21800.020.32998543
CGTATGC21500.020.29974644
GTCACGC21250.020.22193129
ATCTCGT22000.020.04338840
AGTCACG23700.018.89136728
GTATGCC24500.017.81477745
GATCGGA27500.017.1149621
TCGGAAG29650.016.3215073
CGTCTGA29050.016.26286316
ATCGGAA29700.015.9988342
CACGCCA28050.015.96373431
CGGAAGA30800.015.7851794
ACACGTC30700.015.6819213
ACGTCTG30800.015.484920515
CACGTCT32100.015.06805714
Produced by FastQC (version 0.11.5)