FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005727766

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727766
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6022785
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC4543577.543968446491117TruSeq Adapter, Index 9 (100% over 51bp)
GATCGGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC104640.17374022150882026TruSeq Adapter, Index 9 (100% over 46bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)