FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727769

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727769
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7852030
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC1922712.448678876672657TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA230500.041.8797451
ATCGGAA236300.041.0325972
TCGGAAG235000.040.917633
CGGAAGA239250.040.181374
GTATGCC237750.038.59734745
CGTCTGA245150.038.4508916
ACACGTC245300.038.34485213
ACGTCTG246900.038.10547315
TCTCGTA240000.038.08701341
CATCTCG239600.038.0661139
CTCGTAT240750.037.9870542
CACGTCT247950.037.9078214
ATCTCGT241500.037.86907640
CGTATGC243500.037.6961144
GCACACG252150.037.6597211
TCGTATG244200.037.60647643
CACACGT251500.037.5871412
GATCATC247150.037.0074436
CCAGATC251650.036.819233
AGAGCAC260200.036.8057988