FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11162810
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATG2098891.8802523737302703TruSeq Adapter, Index 27 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA255650.042.935341
ATCGGAA264400.041.6926462
TCGGAAG264300.041.683543
CTCGTAT253950.041.3741244
TATCTCG253150.041.34470741
CGTCTGA264250.041.30194516
TCTCGTA254300.041.27293843
ACACGTC263350.041.27223213
CGGAAGA266800.041.2507864
TCGTATG256050.041.18416645
ATCTCGT255600.040.99241642
ACGTCTG267150.040.81149315
CACGTCT267350.040.72205414
CACACGT269350.040.30274212
GCACACG270300.040.2526511
AGAGCAC282950.038.763168
CCAGTCA279950.038.45973626
GAGCACA285800.038.3844879
GAAGAGC289550.038.2803276
CAGTCAC282050.038.1574327
Produced by FastQC (version 0.11.5)