FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727772

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727772
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8028814
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC2843303.541369871066885TruSeq Adapter, Index 4 (100% over 51bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCG226600.2822334656152204TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA366350.040.6901251
TCTCGTA370000.037.9405341
GTATGCC374300.037.8813745
CTCGTAT371200.037.83605642
ATCTCGT373500.037.71146840
AATCTCG374300.037.5707739
CGTATGC377600.037.5283544
ATCGGAA398700.037.4617732
TCGTATG378550.037.41634843
TCGGAAG397400.037.394673
CGGAAGA397550.037.340934
ACCAATC375800.037.29257236
GACCAAT383650.037.11522735
ACTGACC386500.037.0571632
CCAATCT377950.037.02679437
CGTCTGA390700.036.9887616
ACACGTC392200.036.938613
ACGTCTG394050.036.6969115
CACGTCT395050.036.60378614
GCACACG400750.036.47558611
Produced by FastQC (version 0.11.5)