FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727774

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727774
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24745718
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT10200.09.26239644
TCTCGTA11500.06.84611943
TCGTATG15700.06.598499345
CGTATGC9158.2945917E-106.399400745
GATCGGA15550.06.1133071
CGTCTGA21150.05.104820716
ACACGTC21200.05.092781513
ATCTCGT16054.3655746E-115.0454442
ATCGGAA19700.05.0302162
Produced by FastQC (version 0.11.5)