FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9295825
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA10600.028.6537743
CTCGTAT11150.027.44213544
GATCGGA12450.027.341181
ATCGGAA13650.024.888472
TCGTATG12800.024.43198245
ATCTCGT13450.022.91670242
ACACGTC14350.022.73369413
TCGGAAG15450.021.6975983
CGTCTGA15150.021.53323416
CGGAAGA16950.019.7784144
ACGTCTG17550.018.8449115
CACGTCT17850.018.15006414
GCACACG20350.016.14144111
CACACGT21700.015.24093112
AATCTCG21200.014.75138541
AGTCAAC23800.013.80168434
GTCAACA28700.011.68047835
AGAGCAC29500.011.2879378
CAGTCAA29700.011.13568933
CCAGTCA31100.010.9967126
Produced by FastQC (version 0.11.5)