FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727782

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727782
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8071924
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATG252590.31292415538104673TruSeq Adapter, Index 21 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAT37500.032.31404537
CGTTTCG38850.031.88529233
TTCGGAA38800.031.34745636
GTTTCGG39500.031.01848234
CTCGTAT39900.030.72471244
TCGTATG41350.029.9172645
TCTCGTA41100.029.82763943
TTTCGGA41050.029.79239535
AGTCACG45650.029.22148928
GTCACGT43050.029.1051929
ACGTTTC42800.028.83736432
ATCTCGT42950.028.59541142
CACGTTT44550.028.00861231
CGGAATC43000.027.98673238
TCACGTT45400.027.40044830
GATCGGA51950.026.9145681
AATCTCG45300.026.76439741
ATCGGAA54750.025.9904822
TCGGAAG55550.025.7676053
CGTCTGA55150.025.57199916
Produced by FastQC (version 0.11.5)