FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727785

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727785
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16708640
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG1535330.9188838828294823TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCCG191850.039.49849335
CCCGATC190150.039.40258838
TCCCGAT192700.039.2436137
GATCGGA203500.039.200831
GTCCCGA193400.039.15981336
ACCCGTC199600.038.45025632
CACCCGT199800.038.3557431
TCTCGTA196700.038.2389343
ATCGGAA209550.038.2189062
TCACCCG201050.038.1170830
CCGTCCC200400.038.07183534
CTCGTAT197350.038.05599644
CCGATCT197050.037.94294439
TCGTATG199200.037.89270845
TCGGAAG210550.037.885433
CCCGTCC202250.037.8349733
ATCTCGT201150.037.393242
CGGAAGA213650.037.314654
ACACGTC208950.037.20120213
CGTCTGA210750.036.9263816