FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50426159
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTCG57400.031.90042934
CTCGTAT59950.031.18141444
TCTCGTA56800.030.49484343
CGCTCGA60750.030.25239635
TCGTATG67650.027.71522545
CGAATCT62250.027.21007239
ACACGCT73800.025.17773832
GCTCGAA74600.024.72629436
ATCTCGT72000.024.27574342
TCACACG78200.023.87662730
CACGCTC78000.023.67781433
CGTCTGA85100.022.6256316
ACACGTC89850.021.3294713
TCGAATC87150.019.84827238
ACGTCTG108500.018.05696915
CACGTCT115150.016.8775114
GTCACAC127350.015.13778829
CACACGC136750.014.01539331
CACACGT139950.014.01529112
TCGGAAG142500.013.6192693
Produced by FastQC (version 0.11.5)