FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727787

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727787
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8166039
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATG635690.7784557482519984TruSeq Adapter, Index 25 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG86150.036.66369641
CTCGTAT86600.036.47318344
TCTCGTA87050.036.28463443
TCGTATG88350.035.85193345
ATCTCGT89100.035.42455342
GATCGGA101950.032.6949351
TCGGAAG104300.032.1152763
ATCGGAA105300.031.9114442
CGTCTGA105100.031.8717416
GTCACAC101750.031.79563529
ACACGTC105500.031.66560213
ACGTCTG106900.031.31403415
CGGAAGA107550.031.1238754
CACGTCT108450.030.8457414
CTGATAT106700.030.43178235
ACTGATA106950.030.2764934
GCACACG112350.029.7950211
CACACGT112550.029.7620612
CAGTCAC110300.029.61725227
CCAGTCA111900.029.294326
Produced by FastQC (version 0.11.5)