FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727788

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727788
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9077570
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATG1504621.6575140703954914TruSeq Adapter, Index 27 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG193200.039.20625745
TATCTCG193500.038.9150941
CTCGTAT195150.038.74682244
TCTCGTA195650.038.624843
ATCTCGT196650.038.39491342
GATCGGA213850.037.4360541
TCGGAAG220850.036.1515733
ATCGGAA221800.036.1043852
CGTCTGA219600.035.90626516
CGGAAGA222250.035.8529554
ACACGTC220850.035.64192213
ACGTCTG221900.035.4732715
GTCACAT220350.035.19462229
CACGTCT223900.035.1664514
CAGTCAC220450.035.12878827
CCAGTCA221300.034.90236326
GCACACG227250.034.61834711
CACACGT227400.034.61529512
CACATTC224850.034.4062731
TTATCTC220300.034.28310440
Produced by FastQC (version 0.11.5)