FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727794

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727794
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16356714
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG723970.4426133513124947TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA97550.039.1220551
ATCGGAA102250.037.453652
TATCTCG92050.037.12571341
TCGGAAG103350.036.9414143
CTCGTAT93800.036.81569744
GTCACGT97300.036.7182929
TCTCGTA94250.036.63992343
ACACGTC102100.036.46742613
TCGTATG95750.036.41858745
CGTCTGA102800.036.131916
CGGAAGA106250.036.081244
AGTCACG99300.036.00261328
ACGTGGC98750.035.5582832
ATCTCGT97250.035.55742
CGTGGCC98000.035.50885833
ACGTCTG105500.035.24952315
TCACGTG101900.034.75068730
CACGTCT108150.034.44820814
GCACACG110550.033.9451811
CACGTGG106150.033.2278531
Produced by FastQC (version 0.11.5)