FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727795

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727795
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13675678
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATG642970.4701558489458439TruSeq Adapter, Index 21 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG80850.039.14286833
GTTTCGG81650.038.48368534
TCGGAAT80950.038.4280337
TTCGGAA81100.038.4124636
GTCACGT87200.037.87467229
AGTCACG88800.037.72649828
TTTCGGA83150.037.4916835
GATCGGA97200.037.2945061
CGTCTGA97700.036.49788716
ACACGTC98150.036.49099713
TCACGTT90150.036.33515530
TCGGAAG100600.036.3187183
ACGTTTC87900.036.157232
ATCGGAA101750.035.8908542
CTCGTAT86950.035.78333344
CGGAATC85950.035.62512238
AATCTCG86700.035.42074241
ACGTCTG100700.035.41055715
TCGTATG88700.035.3817145
TCTCGTA88250.035.28170843
Produced by FastQC (version 0.11.5)