FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005727796

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005727796
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16027424
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATG398710.24876736274026318TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA60550.037.9796141
GTCACGA57950.036.2604129
ATCGGAA64850.035.737662
TATCTCG57300.035.5722641
TCGGAAG65100.035.3889543
CTCGTAT59500.034.5966444
TCTCGTA59700.034.3675843
ACACGTC65000.034.12747613
CGTCTGA65100.034.04070716
AGTCACG62700.033.9463228
CGGAAGA67850.033.755884
CACGAGT62100.033.7072331
ACGAGTG62350.033.64427632
TCGTATG61700.033.5818245
ATCTCGT62050.032.99419442
ACGTCTG67950.032.67896715
CACGTCT69500.031.98252714
GCACACG71700.031.00129111
TCACGAG69250.030.44015730
CACACGT74550.029.78688212
Produced by FastQC (version 0.11.5)