FastQCFastQC Report
Wed 11 May 2022
EGAF00005729486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47686945
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACTTGGATCTCGTAT3902960.8184546105857693TruSeq Adapter, Index 4 (97% over 37bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1962210.411477397010859No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACTTGGATCTCGTATG1282600.2689625011625299TruSeq Adapter, Index 4 (97% over 36bp)
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGA708250.14852073245623093No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA634280.1330091495691326No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCA615910.12915694221972074No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGG602990.12644760531420077No Hit
CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTT487030.1021306774841626No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)