FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005729488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77475087
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACTTGGATCTCGTAT5787200.7469756052032571TruSeq Adapter, Index 4 (97% over 37bp)
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGG910440.11751390482465673No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA844660.10902343355871288No Hit
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGA807940.10428384546376825No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG779350.043.3238644-45
GTATGCC853000.039.57142646-47
GCCGTCT856400.039.26877650-51
ATGCCGT887150.037.9865848-49
TATGCCG795250.037.4462348-49
CGTATGC819050.036.38712346-47
CTCGTAT851950.034.9685644-45
CGGACTT983750.034.5244732-33
GATCTCG979950.033.44084540-41
CACGGAC1027100.033.02420430-31
TGCCGTC944350.031.64606350-51
ACGGACT946200.031.47709332-33
GTCACGG1078600.031.42573228-29
GCTTGAA1156000.029.46606860-61
AGTCACG1032250.028.9306228-29
TTGGATC1009050.028.69427138-39
TTGAAAA1210200.028.10409762-63
CTTGAAA1077500.027.80177962-63
TCACGGA1120350.026.78437630-31
TGGATCT1236050.026.64853538-39
Produced by FastQC (version 0.11.5)