FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005729490

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729490
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59562039
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCA682170.11453100186848875No Hit
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGA608100.10209522880840262No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTACG97550.026.24243
GTTACGA97950.026.2313524
TTACGAC109050.023.4743655
ACGACTT198850.020.6069431
TACGACT128100.020.020426
GTATCGA81100.017.9781674
GTTACGG414100.017.5240297
TTACGGA420900.017.3653228
AGTTACG425150.017.2031156
TTCGCGC125800.016.6106977
CGATCCG207800.016.58611382-83
CCCGTAT114450.016.0698661
GTTCGAC223150.015.60018228-29
TCGACCG224800.015.52784630-31
TACGGAT489200.015.2225769
ATCGAAG438250.014.9874876
TATCGAA98950.014.8790995
AAGTTCG236550.014.67650526-27
TGTCGAG89750.014.6580475
CCGAAGT509450.014.6033412
Produced by FastQC (version 0.11.5)