FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005729493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59931194
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC8089241.3497545201585672Illumina Single End PCR Primer 1 (96% over 33bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3553570.5929416323659428No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT1750630.29210664483006965No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG647870.10810230144922527No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA893300.045.2005260-61
GCCGTAT895350.045.0439958-59
TCACCCG426450.044.3483123
CGCCGTA908350.043.87290256-57
GGTCGCC936200.043.7659554-55
CCGTATC914450.043.4512358-59
CGGTGGT970250.042.22652450-51
TCTCGGT960300.042.09946-47
GTGGTCG976050.041.96518352-53
CTCGGTG984950.041.74337848-49
CACCCGG455950.041.3639374
TCGGTGG982850.041.22605548-49
CGGCCCG459300.040.5975278
GTATCAT984600.040.48328460-61
TAGATCT1045150.038.92450742-43
TAGTGTA1074300.038.38563538-39
ATCTCGG1087800.037.887346-47
GCTATAG1095550.037.14141534-35
TATAGTG1123150.036.51737636-37
AGATCTC1134650.036.4982644-45
Produced by FastQC (version 0.11.5)