FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005729495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65338101
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT1910390.29238529598526286No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT829510.12695655173694137No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG656960.10054776461899313No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCG537900.037.824443
CACCCGG556100.036.6393784
TAGCATA224550.029.8108799
CGGCCCG724800.028.559198
ACCCGGC750400.027.1517095
CGGACAC511200.023.67802612-13
CTCACCC921100.022.402982
GTAGCAT307350.022.0735178
GACACGG560800.021.75467914-15
CGGACAG569700.021.32022518-19
CCAGTAG320850.020.7873465
CGACCTA69050.020.3644624
CCGGACA529850.019.82763912-13
ACGGACA543600.019.3565418-19
CCCGGAC631500.019.29656810-11
CAGTAGC353350.019.2921546
CCTCACC1121100.019.2905831
CCGGCCC1138700.019.2612677
AACGCGC61200.019.0967969
GCCAGTA351400.019.034694
Produced by FastQC (version 0.11.5)