FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005729504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71371286
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGCGGTTATCTCGTAT7290461.0214836257819426TruSeq Adapter, Index 23 (97% over 37bp)
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGG908690.1273187090954197No Hit
CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCG749190.10497078615060965No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGCGGTTATCTCGTATG744230.10427582879759234TruSeq Adapter, Index 23 (97% over 36bp)
ACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCT729390.10219656123332287No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)