FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005729505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71371286
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTAGCGCTGTGTAGATCT7449661.0437895150158847Illumina Single End PCR Primer 1 (96% over 32bp)
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT2722610.3814713384875817No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG755130.10580305362579569No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT921750.044.5034758-59
CGTATCA921400.044.33680360-61
CGCCGTA914050.041.08502658-59
GGTCGCC1015700.040.6441154-55
CCGTATC941250.039.8795860-61
TCACCCG728500.039.492283
GTGGTCG1055100.038.98934652-53
ATCTCGG1094050.038.6736846-47
CTAGCGC1104300.037.88424332-33
TCTCGGT1011900.037.64528348-49
GTATCAT997500.037.59027562-63
CACCCGG765250.037.533654
GTAGATC1133150.037.27068342-43
CGGTGGT1113500.037.24529350-51
TAGATCT1051700.036.33612444-45
GTGTAGA1160200.036.30483640-41
AGATCTC1178800.035.9883344-45
CTCGGTG1172050.035.887348-49
GTCTAGC1172750.035.60183330-31
TAGCGCT1061700.035.4290734-35
Produced by FastQC (version 0.11.5)