FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005729506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66589633
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCG1271320.19091860740545002No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG1152440.17306597860360637TruSeq Adapter, Index 7 (97% over 34bp)
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGA1064020.15978763541165633No Hit
CTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT1020390.1532355644609124No Hit
CTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGA944310.1418103625830165No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACA924730.13886996493883663No Hit
CTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAAT903810.1357283347694678No Hit
CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTT895250.13444284938467824No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG820470.12321287309092094No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA729410.1095380717896433No Hit
CTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTT684080.10273070584425657No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGG676550.10159989919151528No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG175950.034.1042144-45
TGTTACG184150.033.841473
TATGCCG178000.033.37767846-47
GTTACGA191300.032.6693844
TTACGAC211750.029.6039875
GTCGAGG138100.028.885866
TACGACT222250.028.2694786
TGTGTCG140000.028.1263663
TGTCGAG173700.028.078275
TATCTCG231400.025.99405340-41
CGTATGC232200.025.66868244-45
CTCGTAT253000.023.68053642-43
GCCGTCT258300.023.39704350-51
TTCGTAT248750.023.37946336-37
GTATGCC263550.022.97555246-47
TCGTATC256900.022.85036836-37
TCCGACG57100.022.5487962
TTACGGA318050.021.5168678
CTCAGAC562350.021.2803041
GTTACGG319300.021.1946587
Produced by FastQC (version 0.11.5)