FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005729510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65799705
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12116801.8414672223834436No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1786490.2715042567440082No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTATG1252560.19035951604950205TruSeq Adapter, Index 1 (97% over 35bp)
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGA900940.13692158650255348No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG192400.037.53901744-45
TATGCCG209400.031.33797646-47
GCGATAG249300.029.06033934-35
CGATAGA229100.028.69978334-35
CGTATGC243100.027.16009944-45
GCCGTCT266350.026.89721350-51
GTATGCC270250.026.82165346-47
CTCGTAT264150.025.26546142-43
AGCGATA264350.025.08750732-33
ATGCCGT303150.024.16523648-49
TGTTACG153100.022.6493023
TGTCGAG136750.022.016035
GTTACGA159150.021.9673984
CAGCGAT358650.020.77533332-33
ACCGCGT53650.020.5350286
TTACGAC169900.020.515395
GATCTCG342500.020.07920840-41
TGCCGTC335800.020.06190148-49
GCGCGTT62250.019.5721690-91
TGTGTCG141400.019.3499663
Produced by FastQC (version 0.11.5)