FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005729516

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729516
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36766402
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2235730.608090506109355No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACTTGGATCTCGTAT1360160.36994645274237065TruSeq Adapter, Index 4 (97% over 37bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370770.10084478758623158No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG211150.041.05008744-45
GCCGTCT253650.034.0325850-51
GTATGCC257200.033.77509346-47
TATGCCG212000.032.967448-49
CGGACTT285050.030.61730432-33
GATCTCG273100.029.79861840-41
CGTATGC239500.029.27080346-47
CTCGTAT244200.028.45384444-45
CACGGAC307550.028.19862230-31
TCTCGTA289900.028.06412142-43
ATGCCGT323100.027.16597748-49
GTCACGG350100.025.30762328-29
ATCTCGT264900.025.05602542-43
CCGGTAA36100.023.7947061
ACGGACT295950.023.61579332-33
TTGGATC292550.022.49435438-39
TGGATCT369350.022.27290538-39
TGCCGTC323950.021.80159650-51
GCTTGAA419650.020.89360260-61
TCACGGA341600.020.84138730-31
Produced by FastQC (version 0.11.5)